Admin Table of Contents
- 1 How many fragments does EcoRI make?
- 2 How many EcoRI sites are there in lambda DNA?
- 3 How many fragments will HindIII cut lambda DNA into?
- 4 How many fragments of DNA will remain after the enzyme is used to cut the DNA?
- 5 How many nucleotides does bacteriophage lambda?
- 6 How many fragments would result if the DNA above was digested with EcoRI?
- 7 Which lane shows the fragments produced when the plasmid is incubated with both EcoRI and BamHI?
- 8 How do you cut DNA into fragments?
How many fragments does EcoRI make?
four fragments
After digestion with EcoRI, you obtain four fragments: 1, 2, 3, and 4.
How many EcoRI sites are there in lambda DNA?
six recognition sites
The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease.
How many bases does EcoRI cut?
Cutting with Eco RI will yield fragments of 1.5, 2, and 3.5 kilobases.
How many fragments will HindIII cut lambda DNA into?
6 fragments
The HindIII digest of lambda DNA yields at least 6 fragments suitable for use as molecular weight standards for gel electrophoresis. This is called a ladder.
How many fragments of DNA will remain after the enzyme is used to cut the DNA?
The human genome, for example, contains about 6 × 109 base pairs (bp) in the 23 pairs of chromosomes. Cleavage of human DNA with restriction enzymes that produce about one cut for every 3000 base pairs yields some 2 million fragments, far too many to separate from each other directly.
How many base pairs are in bacteriophage lambda?
48,502 base pairs
In its circular form, the phage genome, therefore, is 48,502 base pairs in length. The lambda genome can be inserted into the E. coli chromosome and is then called a prophage. See section below for details.
How many nucleotides does bacteriophage lambda?
The DNA in its circular form contains 48,502 base-pairs.
How many fragments would result if the DNA above was digested with EcoRI?
3. You have a purified DNA molecule, and you wish to map restriction-enzyme sites along its length. After digestion with EcoRI, you obtain four fragments: 1, 2, 3, and 4.
Where does EcoRI cleave DNA?
GAATTC sites
Summary One popular recombinant DNA tool is the EcoRI endonuclease, which cleaves DNA at GAATTC sites and serves as a paradigm for sequence specific DNA-enzyme interactions.
Which lane shows the fragments produced when the plasmid is incubated with both EcoRI and BamHI?
Gel lane IV
5. Which lane shows the fragments produced when the plasmid was incubated with both EcoRI and BamHI? EcoRI and BamHI would make cuts at the one EcoRI and the three BamHI restriction sites, producing five DNA fragments at 2kb, 4kb, 6kb, and 8kb in length. Therefore, Gel lane IV shows the digest with EcoRI and BamHI.
How do you cut DNA into fragments?
In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences. Different restriction enzymes recognise and cut different DNA sequences.